Ricardo Martínez Rosales*, Ana Campal Espinosa, Amalia Vasquez Arteaga, Sheila Chavez Valdes, Gilda Lemos Perez and Celia del Carmen Crespo Oliva
Regulatory T cells (tregs) has been consider as a potential biomarker in rheumatologic clinical trials as this cell population has been shown to be involved in the autoimmune diseases outcome. Human tregs are defined as CD4+CD25+/ highCD127-/low cells; however its accurate identification by flow cytometry has been argued, especially as a result of different gating strategies. A flow cytometry gating strategy was settled to measure human Tregs from PBMC isolated via density gradient using CPT tubes. With this method we performed a standardized procedure for treg profiling, useful for immunomonitoring in clinicals assessment.
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