Gharieb MM , Magdy Z Matter , El Shobaky Ahmed , Wael taufik and Haffez EE
The Green Synthesized Nanosilver and their Effects on the Multidrug Resistant Bacteria Ribosomal DNA
Green synthesis of reliable and eco-friendly of metallic nanoparticles is an important step in the field of nanotechnology. In this study silver nanoparticles were biosynthesized using Trichoderma sp. These particles were characterized by Transmission Electron Microscope (TEM) and XRD. The results showed well dispersed nanoparticles with size ranges between 0.025 µm to 0.047 µm. The antibacterial activity of the obtained nanoparticles was tested using agar well diffusion method. The results bare that high antibacterial activity against gram positive Staphylococcus aureus, and gram negative Escherichia coli, Bacillus cereus, Proteus, Klebseilla and Pseudomonas aerouginosa. The inhibition zones were 4.2, 2.4, 5.1, 3.0, 4.5, 1.2 cm respectively. Both of Staphylococcus aureus and Escherichia coli were selected and the16S rRNA genes were isolated from treated and non-treated cells with AgNps. The PCR of the 16S rRNA gene were digested with two restriction enzymes. The PCR-FLP results revealed that different DNA band pattern was observed, and distinguished between the treated and nontreated cells. Moreover, the 16S rRNA were subjected to DNA sequence and the sequence analysis revealed that the DNA nucleotides sequence of 16S rRNA genes isolated from the treated Staphylococcus aureus and Escherichia coli cells were different in comparison with the non-treated cells. Thus, we can conclude that biosynthesized nanoparticles can be used as effective antimicrobial agent against some important pathogenic bacteria in addition it can be used in medical applications.
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