Arti Sharma*, Jyoti Parkash, Priya Godara, Nymphaea Arora, Vikash Prashar, Tania Aror, Randeep Singh, Arpita Banerjee and Harish Changotra
Nitric Oxide (NO) is a hetero-nuclear diatomic molecule which is formed from L-arginine through catalytic activity of Nitric Oxide Synthase (NOS). NO is an important messenger molecule known to be involved in dilation of smooth muscles, penile erection, synaptic plasticity. The three isoforms of NOS are nNOS (neuronal NOS), iNOS (inducible NOS), and eNOS (endothelial NOS). The iNOS is coded by NOS2 gene and is known to play an important role in body’s immune response involving inflammatory response, leukocyte mediated microbial destruction. In this study the iNOS sequences was compared among 18 organisms from arthropods to mammals including bacteria. To understand the implication of iNOS the domain as well as phylogenetic analysis was done. The various biochemical, physico-chemical, and nearly two possible transmembrane models were predicted to know more about the functional regions and structural features of iNOS. One deleterious SNP was predicted through literature survey which is known to have harmful impact on the function of protein and may be used for genomic association studies. The 14 different Post-translational Modifications (PTMs) were predicted which might play an important role in influencing protein interaction network. Nearly 8 Transcription Factors (TFs) were predicted in mouse and rat which may directly influence the NOS2 expression. Using different in silico methods we actually made a comparative analysis of the different parameters of iNOS to deeply understand more of its regulatory activities. In conclusion, results obtained from this study may be used to create a link between the regulations of iNOS in various organisms and in future the results might be exploited for the clinical trials to regulate various autoimmune disorders.
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