Reyes-Nava LA, Garduno-Siciliano L, Estrada-de los Santos P, Hernández-Sanchez H, A Arauz J, Muriel P and Rivera- Espinoza Y
Use of bile acids as a selection strategy for lactobacillus strains with probiotic potential
An in vitro model that simulates some gastrointestinal (GIT) tract conditions was used to select gram-positive and catalase-negative bacilli. Then, their capacity to dissociate bile salts conjugated to taurine and glycine was qualitatively and quantitatively evaluated. The in vivo experiments were performed on male Wistar rats. Lactobacillus strains were administered orally using an intubation needle, at a daily dose of 1x109 CFU/mL for 21 days. A high-cholesterol diet was administered from day 14 to 21. Total cholesterol, LDL-cholesterol, HDL-cholesterol, lipid peroxidation, and liver enzymes levels were measured.
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